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Part:BBa_K940001

Designed by: Moyin Odugbemi   Group: iGEM12_Westminster   (2012-09-25)


ALDH2 promoter in a pSB1C3 backbone

ALHDH 2 is an isoform of aldehyde dehydrogenase; which is one of the enzymes involved in alcohol metabolism. Our construct contains the promoter for ALDH2 restricted with EcoR1 and SPe1 and then placed in the pSB1C3 backbone.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 37
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 37
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 37
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 37
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 716
    Illegal BsaI site found at 763
    Illegal BsaI site found at 881
    Illegal BsaI.rc site found at 352


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